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To distinguish between preferential binding of the p40 AUF1 isoform to EBER1 and enhanced crosslinking efficiency of this isoform, we repeated the immunoprecipitation of Flag-tagged p40 AUF1 in the presence of Myc-tagged p40 and p45 isoforms.
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An in vivo interaction of the p40 isoform and EBER1 is supported by the fact that both molecules are found in the same subcellular compartment the p40 isoform localizes to both cytoplasm and nucleus [ Xu et al.
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